Figure 3

Spiperone, ionomycin and thapsigargin inhibit Wnt1- and LRP6-initiated Wnt signaling. HEK293 cells were transfected with a TOPflash reporter and expression plasmids for Wnt1, LRP6 and Dvl as indicated in the figure. After transfection for 24 h, cells were grown with 5 μM spiperone, 2 μM ionomycin, 50 nM thapsigargin and vehicle control for another 24 h. Then luciferase values were determined.