Figure 1

Schematic representation of the DDV for transport of BoNT/A antagonists. The schematic representation ofthe DDV construct without drug. The PDPH linker is bound to one of four possible cysteine (C) sulfhydryl groups on the BoNT/A rHC. It is attached to C454, which normally participates in the disulfide linkage with the LC. Cy3 and Oregon green 488 are bound to O-amino groups of lysine in the rHC and dextran, respectively. The dextran is conjugated to the rHC by a C-N bond in one of the glucose residues. In a functional DDV, multiple drug molecules may be attached to dextran carrier.