Figure 3

The relative expression levels of ApoE, ABCA1, NPC1L1 and protein levels of ApoE in psychotropic drug-exposed cultured human hepatoma (HepG2) cells. Left panel: Gene expression levels in HepG2 cells were exposed to 25 μM of clozapine, olanzapine, haloperidol or imipramine, for 24 hours. The data represents mean values ± SEM (n = 4). Right panel: The protein level of ApoE determined by western blotting in HepG2 cells exposed to 25 μM of the drug (clozapine, olanzapine, haloperidol and imipramine) or vehicle for 24 hours. The data are normalized relative to the level of beta-actin, showing mean values ± SEM (n = 3) of the ratio between the drug- and vehicle-exposed cells. * indicates p < 0.05, ** indicates p < 0.01.