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Table 2 Summary of MTSEA modification data.

From: Conformational changes in α7 acetylcholine receptors underlying allosteric modulation by divalent cations

MUTANT

MTSEA

modification rate

× 103, M-1 S-1 (n)

 

control

+ ACh

+ Ba 2+

M37C

2.4 ± .03 (10)

0.30 ± 0.05 (4)

0.40 ± 0.02 (5)

M40C

16 ± 0.2 (13)

2.2 ± 0.4 (3)

2.8 ± 0.05 (7)

N52C

0.40 ± .08 (9)

1.4 ± 0.04 (7)

0.26 ± 0.02 (7)

E44C

23 ± 0.7 (4)

0.90 ± 0.05 (4)

9.3 ± 0.1(8)

N170Ca

3.3 ± .05 (7)

5.7 ± 0.1 (7)

3.7 ± 0.06 (9)

E172Ca

15 ± 0.4 (5)

3.5 ± 0.1 (5)

7.3 ± 0.3 (6)

M40C/E172Qa

14 ± 0.5 (5)

1.3 ± 0.1 (5)

2.0 ± 0.3 (6)

  1. Second-order rate constants ± standard error for various conditions at each Cys mutant. (n), number of determinations.
  2. (a) Mutants studied using co-expression with human RIC-3 (Halevi et al., 2002).
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BMC Pharmacology

ISSN: 1471-2210