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Table 1 Effects of Ba2+ on ACh evoked currents.

From: Conformational changes in α7 acetylcholine receptors underlying allosteric modulation by divalent cations

mutant

Ach

EC50, μM

(n)

ACh Imax μA

Ba2+ efficacya

 

control

+ 10 mM Ba 2+

   

C115A/L247T

1.8 ± 0.4

0.17 ± 0.02

4

6.9

0.1

M37C

2.0 ± 0.5

0.35 ± 0.12

4

0.92

0.2

M40C

7.7 ± 1.1

1.1 ± 0.1

5

3.0

0.1

N52C

2.6 ± 1.3

ndb

5

0.34

0.6

E44C

7.8 ± 0.4

10.1 ± 0.4

4

5.4

0.01

N170Cc

13 ± 1.7

2.1 ± 0.4

6

1.7

0.03

E172Cc

30 ± 2.7

50 ± 9.2

4

1.3

0.02

M40C/E172Qc

85 ± 6.2

134 ± 11

5

0.7

0.02

  1. ACh dose-response measurements in the absence or presence of Ba2+. EC50 estimates are mean values ± SEM.
  2. (a) Ba2+ efficacy is the fraction of maximal ACh-evoked current that is evoked by 10 mM Ba2+ in the absence of ACh. (b) nd: not determined because Ba2+ was a strong partial agonist. (c) Mutants studied using co-expression with human RIC-3 (Halevi et al., 2002).