Comparison of four different colorimetric and fluorometric cytotoxicity assays in a zebrafish liver cell line

BMC Pharmacology

Table 2 Quality parameters intra- and inter-assay variability and signal-to-noise ratio for the four cytotoxicity assays.

Variability of cytotoxicity assays based on experiments assessing Tam, 4-OHT, and Flus toxicity. Intra-assay variability was calculated based on triplicate wells assessed within one assay and median values were calculated based on three independent experiments. Inter-assay variability was calculated as the deviation of effective concentrations based on each three independent experiments. For both, intra- and inter-assay variability, the coefficient of variance (CV [%]) is shown in the table. The signal-to-noise ratio was calculated for Tam exposure experiments by dividing the absorption or fluorescence in the wells containing untreated or treated cells by the signal in background wells without cells. This was performed for each Tam concentration and median values are shown for low (0–20%) and high (80–100%) cytotoxicity ranges.