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Figure 1 | BMC Pharmacology

Figure 1

From: Comparison of four different colorimetric and fluorometric cytotoxicity assays in a zebrafish liver cell line

Figure 1

Effects of different cell densities. Effects of different cell densities were studied in the four cytotoxicity assays and the linear range determined by linear regression. For all assays 25,000 cells/well was selected to be used as standard cell number in the cytotoxicity tests, which was included in the linear range for all assays. Panel A shows the results for the LDH and MTT assays with the Y-axis indicating absorption with respect to the reference wavelengths, i.e. abs590 with reference at 655 nm for LDH and abs570 with reference at 680 nm for MTT. Panel B shows linearity for the AB and CFDA-AM assays, with the Y-axis indicating the fluorescent units [FU] detected at 530 nm excitation/590 nm emission for AB and 485 nm/535 nm for CFDA, respectively.

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