Figure 1From: Comparison of four different colorimetric and fluorometric cytotoxicity assays in a zebrafish liver cell lineEffects of different cell densities. Effects of different cell densities were studied in the four cytotoxicity assays and the linear range determined by linear regression. For all assays 25,000 cells/well was selected to be used as standard cell number in the cytotoxicity tests, which was included in the linear range for all assays. Panel A shows the results for the LDH and MTT assays with the Y-axis indicating absorption with respect to the reference wavelengths, i.e. abs590 with reference at 655 nm for LDH and abs570 with reference at 680 nm for MTT. Panel B shows linearity for the AB and CFDA-AM assays, with the Y-axis indicating the fluorescent units [FU] detected at 530 nm excitation/590 nm emission for AB and 485 nm/535 nm for CFDA, respectively.Back to article page