Figure 4
Effect of LUF6000 on agonist-induced activation of the human A 1 AR studied with a [35S]GTPγS binding assay. Incubations were started by addition of the membrane suspension (5 μg protein/tube) to the test tubes, and carried out in duplicate for 30 min at 25°C. Results were from 3 independent experiments performed in duplicate. The basal values ranged from 700 to 1000 cpm. The maximal values are typically from 1500 to 1800 cpm.