Figure 3From: Flexible modulation of agonist efficacy at the human A3 adenosine receptor by the imidazoquinoline allosteric enhancer LUF6000Effect of LUF6000 on various human A 3 AR ligands, studied with a [35S]GTPγS binding assay. Incubations were started by addition of the membrane suspension (5 μg protein/tube) to the test tubes, and carried out in duplicate for 30 min at 25°C. The experimental procedures are described in the Materials and Methods section. Results were from 3–5 independent experiments performed in duplicate. The basal values typically ranged from 800 to 1200 cpm. The maximal values are typically from 2000 to 2500 cpm.Back to article page