Figure 2

Blockade of brimonidine effects by RX821002. Either RX821002 or water was administered by i.p. injection 30 min prior to brimonidine. At time 0 the rectal temperature was taken, and the rat was immediately injected with brimonidine or saline. Rectal temperatures were then taken at various times thereafter, and the change in temperature from time 0 was calculated. Adult rats (upper panel; 2 to 10 rats per group). Average baseline temperature was 35 ± 0.2°C. An ANOVA of the change in temperature at the 90 min point indicates a significant difference (P < 0.001) between the 0 and the 50 and 100 μg/kg dose of RX821002 in the presence of brimonidine. However there was no statistical difference between animals receiving neither brimonidine and RX821002 and those receiving RX821002 alone (p > 0.05). Post-natal day 13 rats (lower panel). Number of rats at each dose is given in Table 1, and the average temperature of rats at 0 time was 35.0 ± 0.2°C for adult rats and 33.1 ± 0.2°C for post-natal day 13 rats. Error bars are S.E.M. An ANOVA of the change in temperature at the 90 min point indicates a significant difference (P < 0.01) between the 0 and the 80 μg/kg dose of RX821002 in the presence of brimonidine. However there was no statistical difference between animals receiving both brimonidine and 80 μg/kg RX821002 and those receiving RX821002 alone (P > 0.05).