Pharmacokinetics and transcriptional effects of the anti-salmon lice drug emamectin benzoate in Atlantic salmon (Salmo salar L.)

Table 1 PCR primers and TaqMan MGB probes, Genbank accession numbers, amplicon sizes and fold changes (microarray and qPCR verification) for the studied genes.

Gene	Accession no.	Forward primer (5'-3')	Reverse primer (5'-3')	TaqMan MGB probe	Amplicon size (bp)	Microarray	qPCR
Thrombospondin 4 precursor Day 14	CB509633	GCAGCGGTACTTTAGGTTGGA	ATCAGGGCCCGTTTCTATGA		141	-1.6	-1.7
Purine nucleoside phosphorylase Day 14*	CB514814	GCCCCCTTCATGGGTACAC	AACGCCTGAACGAACGAATG		135	2.1	1.6
CYP2B19 Day 7	CA053315	AGCCTGTGACCTCTCCACAGTAA	CGGCACAAAACCTCCAGAAG		134	-3.2	-2.2
CYP2A5 Day 7	CB491960	AGGTTTGGTGCCGGTGAAA	ATGATGGATTCTTTGCTTTTGGA		125	-3.2	-1.7
Low affinity immunoglobulin epsilon Fc receptor Day 14*	CB511007	CCACTCACAGGGCACATCAA	GTGGTCAGATGGGTCCAGATTT		133	2.8	2.1
NADH-ubiquinone oxireductase chain 1 Day 7	CB488966	GGCCGGCACGAGTAGTCA	GGCAGTGGCACAAACCATTT		133	-3.3	-1.2
Sequestosome-1 Day 14*	CA056074	GGGACAGAAAGAGAAGGCAGTATT	GCCCTGGACACCATCCACTA		131	1.3	1.1
HLA class II histocompatibility antigen, gamma chain Day 14*	CK990815	TTATATGCTGTCCGAAGGCAAA	CCCTCCCCCAAAAAATACACA		141	1.1	1.2
Heat shock protein 70	AJ632154	TCAACGATCAGGTCGTGCAA	CGTCGCTGACCACCTTGAA	CCGACATGAAGCACTGG	141
Glutathione S-transferase pi	BQ036247	ATTTTGGGACGGGCTGACA	CCTGGTGCTCTGCTCCAGTT	TTCTCGACAAAGCTC	81
β-actin	BG933897	CCAAAGCCAACAGGGAGAAG	AGGGACAACACTGCCTGGAT	TGACCCAGATCATGTTT	91
EF1AB	BG933853	TGCCCCTCCAGGATGTCTAC	CACGGCCCACAGGTACTG	CCAATACCGCCGATTTT	59
ARP	AY255630	TCATCCAATTGCTGGATGACTATC	CTTCCCACGCAAGGACAGA	CAAATGTTTCATTGTCGGCG	101