Clofibrate treatment in pigs: Effects on parameters critical with respect to peroxisome proliferator-induced hepatocarcinogenesis in rodents

Table 4 Sequences of specific primers used for RT-PCR

Gene (NCBI Genbank)	Forward Primer	Reverse Primer	Size, bp
ACO (AF185048)	CTCGCAGACCCAGATGAAAT	TCCAAGCCTCGAAGATGAGT	218
bax (AJ606301)	CGAACTGATCAGGACCATCA	ACAGCCCATCTTCTTCCAGA	190
bcl-X_L (NM_214285)	GAAACCCCTAGTGCCATCAA	GGGACGTCAGGTCACTGAAT	196
Catalase (NM_214301)	CAGCTTTAGTGCTCCCGAAC	AGATGACCCGCAATGTTCTC	180
c-fos (Y14808)	CTGACACACTCCAAGCGGTA	CTTCTCCTTCAGGTTGG	209
c-jun (NM_213880)	CAGAGCATGACCCTGAACCT	TTCTTGGGGCATAGGAACTG	200
c-myc (NM_001005154)	AATGTCTTGGAACGCCAGAG	CAACTGTTCTCGCCTCTTCC	204
GAPDH (AF017079)	AGGGGCTCTCCAGAACATCATCC	TCGCGTGCTCTTGCTGGGGTTGG	446
GSH-Px (NM_214201)	CAAGAATGGGGAGATCCTGA	GATAAACTTGGGGTCGGTCA	190
GST (NM_214300)	TTTTTGCCAACCCAGAAGAC	GGGGTGTCAAATACGCAATC	246
p53 (NM_214145)	GCGAGTATTTCACCCTCCAG	TCAGGCCCTTCTCTCTTGAA	199
PPARα (DQ437887)	CAGCCTCCAGCCCCTCGTC	GCGGTCTCGGCATCTTCTAGG	381
SOD (AF396674)	TCCATGTCCATCAGTTTGGA	CTGCCCAAGTCATCTGGTTT	250
TNFα (X57321)	CCCCTGTCCATCCCTTTATT	AAGCCCCAGTTCCAATTCTT	200

Abbreviations: ACO, acyl-CoA oxidase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GSH-Px, glutathione peroxidase; GST, glutathione S-transferase; SOD, superoxide dismutase; TNFα, tumor necrosis factor α.