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Table 4 Total GRK levels are unaltered in SHβ2AR4 cells with catecholamine treatment.

From: The presence of β2-adrenoceptors sensitizes α2A-adrenoceptors to desensitization after chronic epinephrine treatment

 

Catecholamine Treatment*

 

EPI

EPI+P

Prop

NE

GRK3

94 ± 8 (7)

84 ± 15 (7)

80 ± 20 (7)

84 ± 20 (5)

GRK2

101 ± 10 (7)

97 ± 16 (7)

105 ± 14 (7)

83 ± 23 (4)

  1. SHβ2AR4 cells were treated with vehicle (0.1 mM ascorbate), EPI (300 nM), propranolol, EPI + propranolol, or 1 μM NE for 24 hr. Approximately 25 μg of whole cell lysate from each treatment group was resolved by SDS-PAGE through a 10% gel. Immunoreactive bands were normalized to the GAPDH loading control and the GRK/GAPDH ratio was calculated.* Data represent % of expression levels noted in vehicle-treated cells (mean ± s.e.m.); number of independent determinations is given in parentheses following the values.