| Catecholamine Treatment* |
---|
| EPI | EPI+P | Prop | NE |
---|
GRK3 | 94 ± 8 (7) | 84 ± 15 (7) | 80 ± 20 (7) | 84 ± 20 (5) |
GRK2
| 101 ± 10 (7) | 97 ± 16 (7) | 105 ± 14 (7) | 83 ± 23 (4) |
- SHβ2AR4 cells were treated with vehicle (0.1 mM ascorbate), EPI (300 nM), propranolol, EPI + propranolol, or 1 μM NE for 24 hr. Approximately 25 μg of whole cell lysate from each treatment group was resolved by SDS-PAGE through a 10% gel. Immunoreactive bands were normalized to the GAPDH loading control and the GRK/GAPDH ratio was calculated.* Data represent % of expression levels noted in vehicle-treated cells (mean ± s.e.m.); number of independent determinations is given in parentheses following the values.