Figure 2
RT-PCR Products obtained from SH-SY5Y RNA using α 2 -AR subtype selective primers. RT-PCR experiments were performed as described in "Methods" using primer pairs recognizing α2C10/C4 (corresponding to α2A and α2C) and α2C4 (corresponding to α2C) gene products (Table 3). The reactions amplified fragments of the expected size from each set of primers. α2C10/C4 primers amplified 233 bp products from SH-SY5Y mRNA that were sensitive to digestion by Bgl II (specific for the α2A product). Restriction digestion with Bst XI of the 630 bp product of α 2C4 primer amplification gave three fragments of 271, 225 and 78 bp. All reactions were performed in the presence (+) or absence (-) of reverse transcriptase (RT) to rule out the possibility of DNA contamination. Lane M designates the 100 bp ladder; the 500 bp fragment is indicated by an arrow in each panel.