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Figure 8 | BMC Pharmacology

Figure 8

From: Gene expression profiling reveals effects of Cimicifuga racemosa (L.) NUTT. (black cohosh) on the estrogen receptor positive human breast cancer cell line MCF-7

Figure 8

Gene expression levels of cyclin G2 (CCNG2) and estradiol receptor α (ESR1) in MCF-7 cells after 24 h treatment with black cohosh extract (15 μg/ml), the triterpene glycoside actein (20 μM), the triterpene aglycon mixture (30 μM), tamoxifen (10 μM) and estradiol (1 nM). For the treatments with extract, tamoxifen and estradiol, results obtained with microarrays (Array) and real-time RT-PCR (PCR) are shown. For actein (ACT) and the aglycons (AGL) expression levels were determined by real-time RT-PCR. Bars represent gene expression levels as fold changes calculated versus DMSO control. RT-PCR measurements were done at least in triplicate. The data are presented as means ± SD (*p < 0.05, **p < 0.01, ***p < 0.001: gene expression statistically significantly different from DMSO control, calculated by Student's t-test).

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