Figure 8

DPH membrane fluorescent measurements. An example record of a poly-APS response showing the measurements made (red text). Changes in membrane fluorescence were quantified relative to pre-drug application fluorescence. The change of fluorescence intensity was measured for each region of interest as the peak response minus pre-drug fluorescence (Peak-Pre). For each individual region of interest the pre-drug baseline fluorescence decay was measured from two time points prior to the application of any drug, this provided a base change of DPH intensity (ΔB). Subtraction of ΔB from peak pre-drug response (see equation) allowed all responses to be normalised for the decay rate of DPH. Data was finally calculated as a % change of pre-drug baseline, see inset equation. All measurements were made from individual raw records that were analysed without filtering or smoothing.