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Table 1 Comparisons of Kd and Ki values to previously published equilibrium dissociation constants for endogenous and modified CGRP ligands (in nM).

From: Identification of specific calcitonin-like receptor residues important for calcitonin gene-related peptide high affinity binding

Peptide Ligand

Wild Type

Reported Wild Type

L24A

L34A

L24A,L34A

[125I-Tyr]CGRP(8–37)

0.9 ± 0.2

0.2a

9.0

9.0

9.0

CGRP

1.4 ± 0.3

0.4H, 32L a

8.9 ± 3.1

10.8 ± 2.2

9.3 ± 2.8

AcCGRP(19–37)

227 ± 86

933b

3000 ± 1400

4600 ± 1200

2000 ± 1000

CGRP(1–36)

> 10,000

n.d.

> 10,000

> 10,000

> 10,000

CGRP(1–19)

> 10,000

n.d.

> 10,000

> 10,000

> 10,000

  1. aVan Rossum et al., 1994; bRovero et al., 1992;
  2. H and L represent high and low binding affinity values respectively calculated from a complex curve fitting model of the data points.