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Figure 5 | BMC Pharmacology

Figure 5

From: Inhibition of p38 mitogen-activated protein kinase enhances c-Jun N-terminal kinase activity: Implication in inducible nitric oxide synthase expression

Figure 5

Expression of p38α and p38β in J774.2 macrophages. (A) Real time RT-PCR analysis of the p38α, p38β and GAPDH mRNA expression in J774.2 macrophages. Shown is the relationship between the PCR cycle number and the ΔRn which is the normalized reporter signal minus the background noise determined during the PCR cycles. Cells were treated with LPS (10 ng/ml) or vehicle only. Total RNA was isolated after 8 h incubation. Six separate mRNA samples were analysed. (B) Cells were stimulated with LPS. After 24 h, incubations were terminated and immunoblots were run using antibody against p38α. The result is representative of three experiments with similar results.

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