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Figure 4 | BMC Pharmacology

Figure 4

From: Inhibition of p38 mitogen-activated protein kinase enhances c-Jun N-terminal kinase activity: Implication in inducible nitric oxide synthase expression

Figure 4

Effect of SB220025 on iNOS mRNA degradation. Cells were stimulated with LPS (10 ng/ml) 1 h before addition of SB220025 (0,5 μM). Actinomycin D (actD) (0,5 μg/ml) was added to cells 6 h after LPS. Incubations were terminated at the indicated time points after LPS and total RNA was isolated. iNOS and GAPDH mRNA was measured by real time RT-PCR. iNOS mRNA levels were normalized against GAPDH. Values are mean ± S.E.M. (n = 3).

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BMC Pharmacology

ISSN: 1471-2210