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Figure 3 | BMC Pharmacology

Figure 3

From: Inhibition of p38 mitogen-activated protein kinase enhances c-Jun N-terminal kinase activity: Implication in inducible nitric oxide synthase expression

Figure 3

Effect of p38 MAPK inhibitor SB220025 on LPS-induced iNOS expression. (A) Cells were stimulated with LPS 1 h before addition of SB220025. After 24 h, incubations were terminated and immunoblots were run using antibody against iNOS and actin. The result is representative of three experiments with similar results. (B) Cells were stimulated with LPS 1 h before addition of SB220025. Incubations were terminated at indicated time points and extracted total RNA was subjected to real time RT-PCR. iNOS mRNA levels were normalised against GAPDH. Values are mean ± S.E.M. (n = 3). *P < 0.05, ***P < 0.001 compared with cells treated with LPS only.

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BMC Pharmacology

ISSN: 1471-2210