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Figure 10 | BMC Pharmacology

Figure 10

From: Inhibition of p38 mitogen-activated protein kinase enhances c-Jun N-terminal kinase activity: Implication in inducible nitric oxide synthase expression

Figure 10

Effect of cycloheximide (CHX) on LPS-induced JNK activation and iNOS mRNA expression. (A) Cells were stimulated with LPS 1 h before addition of different concentrations of CHX. Incubations were terminated 2 h after LPS and parallel immunoblots were run from same cell lysates using antibodies against Thr183/Tyr185 phosphorylated JNK (pp54 and pp46), total JNK (p54 and p46). The result is representative of two experiments with similar results. (B) Cells were stimulated with LPS 1 h before addition of CHX. Incubations were terminated at indicated time points and extracted total RNA was subjected to real time RT-PCR. iNOS mRNA levels were normalised against GAPDH. Values are mean ± S.E.M. (n = 3). (C) Cells were stimulated with LPS 1 h before addition of tested compounds. Incubations were terminated 10 h after LPS and extracted total RNA was subjected to real time RT-PCR. iNOS mRNA levels were normalized against GAPDH. Values are mean ± S.E.M. (n = 3). ***P < 0.001.

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BMC Pharmacology

ISSN: 1471-2210