Figure 5

Functional characterisation of LE404 (■) and LE410 (●) at hD ₁ (A, B, C) and hD _2L receptors (D, E, F). A Inhibition by LE404 and LE410 of 100 nM SKF38393-stimulated accumulation of intracellular [cAMP]. Data shown are means ± SEM of at least four determinations assayed in triplicate. B Inhibition by LE404 and LE410 of 100 nM SKF38393-stimulated increase in intracellular [Ca²⁺]. Data shown are means ± SEM of at least four determinations assayed in triplicate. C Inhibition by LE404 and LE410 of G-protein activation obtained by 1 μM dihydrexidine-stimulation. Data shown are means ± SEM of two independent experiments assayed in duplicate. D Inhibition by LE404 and LE410 of 100 nM quinpirole-stimulated decrease of intracellular [cAMP] in the presence of 10 μM forskolin. Data shown are means ± SEM of at least four determinations assayed in triplicate. E Inhibition by LE404 and LE410 of 30 nM quinpirole-stimulated increase in intracellular [Ca²⁺]. Data shown are means ± SEM of at least four determinations assayed in triplicate. F Inhibition by LE404 and LE410 of G-protein activation obtained by 10 μM quinpirole-stimulation. Data shown are means of two independent experiments assayed in duplicate.