Figure 8

Application of c.Sg increases K⁺-evoked calcium flux into cultured DRG neurones. A) Bar chart showing a significant enhancement of the K⁺-evoked Ca²⁺ flux induced by c.Sg compared to the control (Con.). B) Bar chart showing mean peak Ca²⁺ transient amplitudes evoked by K⁺ under control conditions (Con.) and in the presence of c.Sg. C) Bar chart showing mean values for the width 50% of the Ca²⁺ transient evoked under control conditions (Con.) and in the presence of c.Sg. For A, B and C mean values ± SEM are shown from 17 neurones (***P < 0.005). D) Example trace of Ca²⁺ transients showing the increase in amplitude in a single neurone exposed to c.Sg. E) Example trace showing enhancement in Ca²⁺ transient duration in a single neurone exposed to c.Sg. F) Trace demonstrating that application of c.Sg can cause K⁺-evoked Ca²⁺ flux in a neurone that previously showed no K⁺-evoked Ca²⁺ flux. G) Example trace showing that c.Lobo has a similar effect to c.Sg. In all traces (D to G) the period of stimulation with KCl (30 mM) is shown by thick bars and the period of application of crude samples from soft corals is shown with thin bars.