Figure 3

PKC isoform expression as determined by Western blot. 4D/WT and D/v-src cells were cultured in media supplemented with 3 μM DHA or AA for 24 h. Fresh media with/without the fatty acids and 10μM araC was added. Cell lysates containing 30 μg protein/lane were loaded and separated on 10% SDS-PAGE gels. Gels were transferred and probed as described in the Materials and Methods. Densitometric values are means ± SEM of 3 – 4 separated experiments. Bars with different letters within the same PKC isoform and cell type are significantly different (Fisher's protected LSD test).