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Figure 3 | BMC Pharmacology

Figure 3

From: Differential modulation of microglia superoxide anion and thromboxane B2 generation by the marine manzamines

Figure 3

Differential effects of manzamines A, B, C, D, E and F on PMA and OPZ-stimulated O 2 - generation by LPS-activated rat neonatal microglia. Rat neonatal microglia (200,000 cells/well) were activated with LPS (0.3 ng/mL) for 17 hours. Manzamines were added 15 min before stimulation with either PMA (1 μM) or OPZ (0.5 mg/mL). After 70 min, agonist-triggered O2- release was determined as described in Materials and Methods. LDH release, indicator of cytotoxicity, was measured as described in Materials and Methods. Data are expressed as percentage of control O2- release triggered by PMA (MZA: 13.8 ± 1.9 nmoles O2-/70 min; MZB, C, D, E, F: 10.8 ± 0.6 nmoles O2-/70 min), or OPZ (9.4 ± 0.7 nmoles O2- /70 min). Data show mean ± SEM of indicated number (n) of experiments. * P < 0.05, ** P < 0.01.

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