Figure 2

Differential effects of manzamines A, B, C, D, E and F on PMA and OPZ-stimulated TXB ₂ generation by LPS-activated rat neonatal microglia. Rat neonatal microglia (200,000 cells/well) were activated with LPS (0.3 ng/mL) for 17 hours. Manzamines were added 15 min before stimulation with either PMA (1 μM) or OPZ (0.5 mg/mL). After 70 min, agonist-triggered TXB₂ was measured as described in Materials and Methods. LDH release, indicator of cytotoxicity, was determined as described in Materials and Methods. Data are expressed as percentage of control TXB₂ release triggered by either PMA (MZA: 1,423 ± 439 pg TXB₂ /70 min; MZB, C, D, E, F: 3,279 ± 281 pg TXB₂ /70 min), or OPZ (4,504 ± 308 pg TXB₂ /70 min). Data show mean ± SEM of indicated number (n) of experiments. * P < 0.05, ** P < 0.01.