Figure 2
From: Cross-talk between phosphatidic acid and ceramide during ethanol-induced apoptosis in astrocytes
DNA fragmentation in astrocytes after treatment with ethanol. Astrocytes were incubated with the compounds given: lane 1, control (serum-free medium); lane 2, serum-containing medium; lane 3, C2-ceramide (50 μM) in serum-free medium; lanes 4–7, ethanol in serum-free medium (concentrations and times as given); lane 8, size markers. After incubation, cells were lysed, DNA was purified, separated on a 3 % agarose gel and stained with ethidium bromide. The experiment was repeated three times with identical results.