Figure 5

Inhibition of oxotremorine-induced salivation. Mice were anesthetized with urethane (1.5 g/kg, i.p.; 1 g/ml). Each mouse was then treated with a single i.p. injection of one of the test agents (atropine sulfate, AT; diphenhydramine, DPH; methoctramine, MOT; 1,1-dimethyl-4-diphenylacetoxypiperidinium iodide, 4-DAMP; desloratadine, DL). Each mouse was then placed prone and head-down on a plexiglass plate inclined at 10° and covered with a sheet of Whatman no. 3 MM filter paper. Fifteen minutes after administration of the test agent, a 0.5 mg/kg dose of oxotremorine was administered in a volume of 1 μl/g. Every five minutes for 30 minutes, each mouse was moved up the incline. Salivation production was measured immediately following the move at the end of each collection period by measurement of the circumference of the moist area of filter paper immediately beneath each mouse's mouth. The sum of the 6 collection periods is listed as Total Salivation Score. Numbers in parenthesis represent the number of animals in each group and asterisk denotes statistical significance (P < 0.05) vs. control.