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Figure 3 | BMC Pharmacology

Figure 3

From: Resistance to gemcitabine in a human follicular lymphoma cell line is due to partial deletion of the deoxycytidine kinase gene

Figure 3

Effect of gemcitabine (0.3 μM) on apoptosis and protein expression of PARP and pro- and antiapoptotic members of the Bcl-2/ Bax family. (A) Flow cytometric detection of the Annexin-V staining in RL-7 and RL-G cells measured at different time intervals after incubation with gemcitabine 0.3 μM (B) PARP cleavage as detected by western blot after gemcitabine treatment at different time intervals (C) Bcl-2 and Bax expression by western blot. RL-7 and RL-G cells were treated with gemcitabine 0.3 μM for 24 and 72 h and subsequently lysed, subjected to SDS-PAGE, and immunoblotted with the corresponding monoclonal antibody as described in Material and Methods. The experiments shown are representative of two performed. C: control.

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