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Figure 8 | BMC Pharmacology

Figure 8

From: 15-deoxy-delta 12, 14-Prostaglandin J2 prevents reactive oxygen species generation and mitochondrial membrane depolarization induced by oxidative stress

Figure 8

Prevention of tBH-induced mitochondrial membrane depolarization by 15d-PGJ 2 . Cells were pretreated with serum-free medium (control) or with 1 μM 15d-PGJ2 (prepared in serum-free medium) overnight, then with 400 μM, 500 μM or 600 μM tBH (without 15d-PGJ2) for 4 hours. The 545/595 emission intensity ratio of each treatment was determined. Results indicated that tBH caused an increase of the 545/595 emission intensity ratio, which was completely prevented by 15d-PGJ2 pre-treatment. Preliminary results indicated that tBH treatment for 4 hours was required to see a significant increase of 545/595 emission intensity ratio (data not shown).

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