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Figure 6 | BMC Pharmacology

Figure 6

From: 15-deoxy-delta 12, 14-Prostaglandin J2 prevents reactive oxygen species generation and mitochondrial membrane depolarization induced by oxidative stress

Figure 6

Prevention of tBH-induced cytotoxicity by 15d-PGJ 2 . (A): Cells plated in 96-well plates were pre-treated with serum-free medium (solid circles) or 1 μM 15d-PGJ2 (prepared in serum-free medium, open circles) overnight, then with various concentrations of tBH (without 15d-PGJ2) for one day. The viability of each treatment was determined by the MTT viability assay. Results indicated that 15d-PGJ2 pretreatment greatly reduced tBH cytotoxicity. (B): Cells were pre-treated with various concentrations of 15d-PGJ2 overnight followed by 450 μM tBH (without 15d-PGJ2) for one day, then the viability of each treatment was determined. Results indicated that 15d-PGJ2 prevented tBH induced cell death in a dose-dependent manner. (C): Cells were treated with serum-free medium (control), 1 μM 15d-PGJ2, 450 μM tBH or 1 μM 15d-PGJ2 overnight pre-treatment followed by 450 μM tBH for one day (15d-PGJ2 + tBH). Results indicated that 15d-PGJ2 greatly prevented tBH-induced cell death. Bar: 50 μm.

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BMC Pharmacology

ISSN: 1471-2210