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Figure 2 | BMC Pharmacology

Figure 2

From: Characterisation of cytotoxicity and DNA damage induced by the topoisomerase II-directed bisdioxopiperazine anti-cancer agent ICRF-187 (dexrazoxane) in yeast and mammalian cells

Figure 2

Analysis of gene expression by real time PCR. Real-time PCR was used to determine the expression of the DNA-damage inducible genes RNR3, HUG1, RAD51, and RAD54 by using the 2-ΔΔCt method. Gene expression was normalized to that of the actin gene ACT1. It can be seen that exposure of yeast cells to m-AMSA results in higher levels of induction of transcription of these four genes than exposure to ICRF-187 when the two drugs are applied at equitoxic concentrations. Error-bars represent SEM of two independent experiments each performed in duplicate.

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