Figure 1

Inhibitory effect of different concentrations of aqueous and macerated extracts from Nigella sativa, diltiazem, and saline on pre-contracted tracheal chains of guinea pigs. group 1 (methacholine induced contraction of guinea pig tracheal chains in the presence of ordinary Krebs solution, blue colour), group 2 (methacholine induced contraction of guinea pig tracheal chains in the presence of calcium free Krebs solution, green colour) and group 3 (KCl induced contraction of guinea pig tracheal chains in the presence of ordinary Krebs solution, red colour). (a) low concentration, (b) medium concentration, and (c) high concentration, for diltiazem only 2 concentrations (low and medium) were examined. Statistical differences between the results of group 1 with those of group 2 and 3; NS: non significant difference *: p < 0.05, **: p < 0.01, ***: p < 0.001. Statistical differences between the results of group 2 with those of group 3, +: p < 0.05, ++: p < 0.01, +++: p < 0.001. Aqueous and macerated extracts did not show any inhibitory effect in group 3 experiments compared to the effect of saline.