Figure 2

Hydrolysis of DMF to MMF by various types of blood cells. Monocytes/lymphocytes, granulocytes, and erythrocytes were purified from blood of healthy volunteers using centrifugational techniques. Next, the various cell types were resuspended in PBS pH 7.4 to concentrations present in whole blood, e.g. 1 × 10⁶/mL monocytes/lymphocytes (A), 5 × 10⁶/mL granulocytes (B) and 5 × 10⁹/mL erythrocytes (C), and then DMF was added to a final concentration of 2 mg/L. At various intervals thereafter samples were collected and the MMF (squares) and DMF (circles) concentrations were measured using HPLC. Results are a representative experiment of 3–4 independent experiments.