Figure 1
From: The FK506 binding protein 13 kDa (FKBP13) interacts with the C-chain of complement C1q
Interaction of C1q-C and FKBP13 in the protein complementation assay. INS-1 cells were transfected with pCMVSPORT-Bla[1]M182TlinkBla[2] (control), or were cotransfected with pCDNA3.1Zeo+/F [I]M182T-15-Zip and pCDNA3.1Zeo+/Zip-15-F[2] (Zip+Zip), or were cotransfected with pCMVSPORT-Bla[1]M182TlinkC1q-Cw/oS and with pCDNA-FKBP13linkBla[2] (13+C1q-C). As a negative control cells were transfected with pCDNA-FKBP13linkBla[2] (sham). After lysis of the cells, β-lactamase activity was measured by hydrolysis rates of nitrocefin. The insert resembles the time dependent effect. The absorbances at 490 nm after 90 min of three independent experiments (mean ± S.E.M.) are shown. The control was normalized to 100 %.