Figure 3

Glycine receptors expressed in different expression systems have similar 'functional' strychnine binding. Cells were pre-treated for 30 seconds with strychnine alone then exposed to a strychnine admixture with an EC₅₀ concentration of glycine. (A) Strychnine-mediated inhibition of glycine-gated currents of GlyRα₂ homomers expressed in HEK 293 (□; IC₅₀ = 78.2 ± 13.5 nM) and L-cells (■; 33.1 ± 6.3 nM). (B) Strychnine-mediated inhibition of glycine-gated currents of GlyRα₂β heteromers expressed in HEK 293 (○; 37.9 ± 7.9 nM) and L-cells (●; 23.2 ± 4.6 nM). (C) Functional K_B values were calculated from IC₅₀ values for individual cells using the Cheng-Prusoff relationship and glycine affinity/Hillslope data represented in Figure 1. Average K_B values are shown for each subunit combination in the two expression systems. There was no significant effect of subunit compositions or expression system.