Skip to main content
Figure 2 | BMC Pharmacology

Figure 2

From: Extrinsic factors regulate partial agonist efficacy of strychnine-sensitive glycine receptors

Figure 2

Relative expression levels does not influence taurine efficacy. (A) HEK and L-cells were co-transfected with the GlyRα2 subunit and GFP. Relative expression levels of α2-protein were examined using western blot analysis of total lysate from equal numbers of GFP+ HEK and L-cells. α2 protein was 4- to 5-fold greater in GFP+ HEK cells than in GFP+ L-cells. (B) Maximal glycine conductance across all experiments was significantly lower in L-cells compared to HEK cells, although only by about 2-fold. This may indicate that a significant amount of α2 protein in HEK cells (A) is present in a non-functional form or not associated with the plasma membrane. (C) Glycine current density vs. taurine efficacy in different cell lines expressing α2 (open symbols), α2β glycine receptors (closed symbols), and in isolated neurons from the adult rat basolateral amygdala. The correlation coefficient between glycine current density and taurine efficacy was 0.14 and was not significantly greater than zero (P >> 0.05). There was also no correlation (R2 = 0.01 to 0.3) between glycine current density and taurine efficacy when comparing individual cells within each of these systems.

Back to article page