Figure 8
The GABAB receptor antagonist CGP52432 had no effect on the actions of pregabalin. A) Bar chart showing the inhibitory effects of 2.5 μM pregabalin in the presence of 10 μM CGP52432 (PGB & CGP) on Ca2+ current amplitude measured at the peak inward current (Peak) and at the end of a 100 ms voltage step command to 0 mV (End). The inset traces show the inhibition of the Ca2+ current produced by 3 minutes application of pregabalin and CGP52432 (PGB + CGP) and partial recovery 5 minutes after removal of the drug perfusion pipette. B & C) Show example records of 25 μM pregabalin in the presence of 10 μM CGP52432 (PGB + CGP) modulating Ca2+ flux evoked by 30 mM KCl. The open bars show the period of stimulation with K+ and the filled bar the application of pregabalin in the presence of CGP52432 (PGB +CGP).