Figure 6
Pregabalin and gabapentin do not have additive actions on cultured DRG neurones. A) Bar chart showing the mean Ca2+ current amplitude recorded at the peak of the inward current (Peak) and end of a 100 ms voltage step command (End). Data is shown for measurements made under control conditions (Control), after 3 to 5 minutes application of both pregabalin (2.5 μM) and gabapentin (2.5 μM) (PGB + GBP) and after 5 minutes recovery (Recovery). B) Bar chart showing normalised data from Ca2+ imaging experiments in which simultaneous application of both pregabalin (25 μM) and gabapentin (25 μM) (PGB & GBP) reversibly attenuated the total Ca2+ flux. C) Record of K+-evoked Ca2+ transients, showing the reversible inhibition produced by 25 μM pregabalin and 25 μM gabapentin (PGB + GBP, filled bar).