Figure 3

Pregabalin produced mixed actions on Ca²⁺ influx evoked by 30 mM K⁺. A) Bar chart showing inhibition of Ca²⁺ influx by pregabalin (25 μM). Data for the total Ca²⁺ fluxes was normalised with respect to the first control response to K⁺ (Ctrl). The second and third responses were obtained in the presence of pregabalin (PGB) and after washing away the pregabalin (Rec). B) Record of K⁺-evoked Ca²⁺ transients showing partially reversible inhibition produced by 25 μM pregabalin (PGB). The period of stimulation is shown with open bars and PGB application with the filled bar. C) Bar chart showing enhancement of Ca²⁺ influx by pregabalin (25 μM). Data for the total Ca²⁺ fluxes were normalised with respect to the first control response to K⁺ (Ctrl). The second and third responses were obtained in the presence of pregabalin (PGB) and after washing away the pregabalin (Rec). D) Record of K⁺-evoked Ca²⁺ transients showing reversible enhancement produced by 25 μM pregabalin (PGB).