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Figure 14 | BMC Pharmacology

Figure 14

From: A study comparing the actions of gabapentin and pregabalin on the electrophysiological properties of cultured DRG neurones from neonatal rats

Figure 14

Pertussis toxin pre-treatment and intracellular (Rp)-cAMP prevented enhancement of K+ current by pregabalin. A) Bar chart showing the mean amplitude of K+ current recorded from DRG neurones pre-treated with pertussis toxin for 16–18 hours with 500 ng/ml (PTX Control) and after application of 250 μM pregabalin (PTX PGB), long term, up to 15 minutes monitoring of the current. B) Traces showing outward K+ currents recorded from a DRG neurone pre-treated with pertussis toxin, prior to pregabalin application (PTX Control) and 10 minutes after application of 250 μM pregabalin. C) Bar chart showing mean data obtained from neurones containing (Rp)-cAMP (30 μM), which was applied to the intracellular environment via the patch pipette solution. Data shows the mean K+ current amplitude recorded 1 minute and 5 minutes after entering the whole cell recording configuration (1 min; 5 min), after 5 minutes application of 250 μM pregabalin (PGB) and 10 minutes after removal of the pressure ejection pipette containing pregabalin. D) Traces from a single experiment showing the outward K+ currents at 1 and 5 minutes after entering the whole cell recording configuration and allowing entry of 30 μM (Rp)-cAMP in the DRG neurones. Also shown are the K+ current inhibited by 5 minutes application of 250 μM pregabalin (PGB) and the recovery of the K+ current after the pressure ejection pipette containing pregabalin was removed. Intracellular (Rp)-cAMP prevented the delayed long-term enhancement of the K+ current evoked by pregabalin.

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