Figure 13

Intracellular application of pregabalin enhanced K⁺ currents in cultured DRG neurones. For these experiments pregabalin was included in the KCl-based patch pipette solution at a concentration of 250 μM. A) Bar chart showing the mean amplitudes of the K⁺ current recorded immediately after entering the whole cell recording configuration (Initial I_K) and the maximum outward current recorded within 16 minutes of entering the whole cell recording configuration (Max I_K). B) Traces of the first K⁺ current recorded using a patch pipette solution containing 250 μM pregabalin (Initial) and from the same cell the maximum outward K⁺ current recorded with intracellular pregabalin (Max). C) Bar chart showing control data recorded from neurones not exposed to pregabalin. Illustrated are the mean amplitudes of the K⁺ current recorded immediately after entering the whole cell recording configuration (Initial I_K) and the maximum K⁺ outward current recorded within 16 minutes of entering the whole cell recording configuration (Max I_K). D) Traces of the first K⁺ current recorded using the standard KCl-based patch pipette solution (Initial) and from the same cell the maximum outward current recorded under control conditions, within 16 minutes of entering the whole cell recording configuration (Max).