Figure 4

Effect of SBTI on the proteolytic activity in supernatants of tc-LIG-stimulated mast cells. Supernatants from PMC stimulated by tc-LIG, tc-OLR, and Ag for 8 hr were incubated with or without SBTI (1 mg/mL) before 150 pg/mL of TNF was added and % degradation calculated. Values indicate proteolytic activity after the subtraction of spontaneous release (17 % ± 7). Star indicates statistically significant difference from spontaneous (p < 0.05, n = 4–5). (Mean ± SEM).