Figure 3
PAR-2ap, PAR-2cp compound 48/80 and Ag-mediated release of proteolytic activity from mast cells. (A) Supernatants from PMC treated with tc-LIG (10 μM), tc-OLR (10 μM), or Ag (10 We/mL) for 8 hr were incubated with 150 pg/mL of TNF. Proteolytic activity was calculated as % degraded TNF according to the formula given in Methods section. Values in the graph indicate proteolytic activity after the subtraction of activity released by sham-treated cells (17 ± 7 %). (B) TNF-degrading proteolytic activity released from PMC by various doses of tc-LIG (20 min). Values in the graph indicate proteolytic activity after the subtraction of activity released by sham-treated cells (22 ± 5 %). (C) TNF-degrading proteolytic activity released by SLI (PAR2-ap, 40 μM), LSI (PAR2-cp, 40 μM) and tc-LIG (10 μM) treated PMC (20 min). Values in the graph indicate proteolytic activity after the subtraction of activity released by sham-treated cells (23 ± 7 %). Values are shown as "mean ± SEM" (n = 3–5). Star indicates statistically significant difference from spontaneous (p < 0.05).