Figure 1

Release of RMCP-1, -5 and CPA from PMC following activation with tc-LIG (PAR-2ap), compound 48/80 and Ag. (A) Supernatants from tc-LIG (10 μM), Ag (10 We/mL) and sham-treated (spon) mast cells were concentrated (10 ×) and Western blot analysis preformed for CPA, RMCP-1 and RMCP-5. Left panel shows Coomassie blue staining of the same gel and right panel Western blot with normal rabbit serum as a negative control. (B) Release of RMCP-1, RMCP-5 and CPA following 20 min and 8 h activation of PMC with tc-LIG (10 μM). (C) Dose response for the release of RMCP-1, -5 and CPA by tc-LIG-stimulated or compound 48/80-stimulated PMC. In all cases representative blots from three experiments with similar results are shown.