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Figure 9 | BMC Pharmacology

Figure 9

From: A homotropic two-state model and auto-antagonism

Figure 9

Examples of fitting HOTSM to experimental data sets taken from indicated sources. The general constraints for fitting is that all seven parameters are positive and a = b. Panel A: additional conditions for a fit to the data set of Winding & Bindslev [18] are: L = 1/100 and AS = AM; which yields a = b = 93.7 ± 11.8, c = 0.677 ± 0.295, d = 1.05 ± 1.18·10-4, and AS = AM = 0.329 ± 0.069. Panel B: additional conditions for a fit to the Kinet et al [38] data are: L = 1/100 and AS = AM; yielding a = b = 54.2 ± 2.7, c = 5.03 ± 3.25 ·10-3, d = 5.20 ± 2.11·10-3, and AS = AM = 7.81 ± 1.39. Panel C: the convex bell-shaped data set of Accomazzo et al [Fig 1A in 39] has the following conditions added to the general constraints: L = 0.1765 and AS = AM; yielding a = b = 3.48 ± 0.16, c = 3.79·10-4 ± 2.202·10-3, d = 0.634 ± 0.633, and AS = AM = 0.496 ± 0.129. Panel D: the reverse bell-shaped data set from Accomazzo et al [Fig 1D in 39] has the following conditions added to the general constraints: L = 0.2658, a·b·d = 1.938, AS < 1, and AM > 1. The obtained fitted parameters are: a = b = 0.532 ± 0.030, c = 0.337 ± 0.176, AS = 1.47 ± 0.79 ·10-2, and AM = 68.3 ± 36.2. From the fitted value for a and b, parameter d may be calculated: d = 1.938/(0.532)2 = 6.85.

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