Table 2 Specific primers for the human P2X1, P2Y1, P2Y2, P2Y4 and P2Y6 receptors were constructed based on published nucleotide sequences [21–25], as follows.
From: Potent P2Y6 receptor mediated contractions in human cerebral arteries
Primer | Nucleotide sequence | Product length × (base pairs) |
|---|---|---|
P2X1 forward | 5'-GTTTGGGATTCGCTTTGA-3' | |
P2X1 reverse | 5'-GGCTGAGAGGGTAGGAGAC-3' | 383 |
P2Y1 forward | 5'-ACGGGCTTCCAGTTTTAC-3' | |
P2Y1 reverse | 5'-CCAAGGGGACACAGAACA-3' | 550 |
P2Y2 forward | 5'-CTCTGCTTCCTGCCATTC-3' | |
P2Y2 reverse | 5'-GCACAAGTCCTGGTCCTCT-3' | 432 |
P2Y4 forward | 5'-CAGCACCAAAGGGACCAC-3' | |
P2Y4 reverse | 5'-GCTTGCCACCACCACAGA-3' | 530 |
P2Y6 forward | 5'-ACAGGCATCCAGCGTAAC-3' | |
P2Y6 reverse | 5'-CGGACACAATGGCAAATA-3' | 526 |