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Table 2 Specific primers for the human P2X1, P2Y1, P2Y2, P2Y4 and P2Y6 receptors were constructed based on published nucleotide sequences [2125], as follows.

From: Potent P2Y6 receptor mediated contractions in human cerebral arteries

Primer Nucleotide sequence Product length × (base pairs)
P2X1 forward 5'-GTTTGGGATTCGCTTTGA-3'  
P2X1 reverse 5'-GGCTGAGAGGGTAGGAGAC-3' 383
P2Y1 forward 5'-ACGGGCTTCCAGTTTTAC-3'  
P2Y1 reverse 5'-CCAAGGGGACACAGAACA-3' 550
P2Y2 forward 5'-CTCTGCTTCCTGCCATTC-3'  
P2Y2 reverse 5'-GCACAAGTCCTGGTCCTCT-3' 432
P2Y4 forward 5'-CAGCACCAAAGGGACCAC-3'  
P2Y4 reverse 5'-GCTTGCCACCACCACAGA-3' 530
P2Y6 forward 5'-ACAGGCATCCAGCGTAAC-3'  
P2Y6 reverse 5'-CGGACACAATGGCAAATA-3' 526