Figure 4

Adrenergic agonist competition with [³H]DHA binding to membranes prepared from HEK293 cells cultured in 10% FBS that had been infected with AAV-β₂AR/EGFP. Membranes were incubated at 30°C for 20 minutes with [³H]DHA and increasing concentrations of either (-)-isoproterenol (circles), (-)-epinephrine (squares), or (-)-norepinephrine (triangles). These data were representative of four separate experiments.