Table 5 Transgene expression in breast cancer cell lines. These values are determined in the same experiments as depicted in figures 10 and 11. A: Infectibility of the cell lines as defined by the percentage of adenovirus DNA seeded that was detected in the cells 2 days after infection B: the transcription efficiency is defined by the ratio of the mRNA values divided by the number of adenoviral copies in the cells. C: The translation efficiency is the ratio of the luciferase activity / luciferase mRNA. D: Luciferase activity in 10³ RLU / mg protein. The absolute values are indicated in italic. The values relative to the ZR 75.1, which was arbitrary set as reference are indicated in plain text.

1

7

1.1

6

1.0

6

1.1

6

1.0

6.7

1.1

6.0

1

10

6

1.2

5

1.1

6

1.2

4

0.8

4.8

1.0

4.9

1

100

9

1.2

9

1.2

9

1.3

8

1.0

7.9

1.1

7.3

1

Average

1.2

1.1

1.2

1.0

1.1

1

SD

0.0

0.1

0.1

0.11

0.1

B

Transcription efficiency (mRNA / Ad DNA)

MOI

Cell lines

EVSA-T

CAMA-1

MCF-7

T47 D

SKBR-3

ZR 75.1

1

213

55

71

18

27

6.8

9

2.2

9.5

2.4

3.9

1

10

176

61

49

17

19

6.6

9

3.2

8.0

2.8

2.9

1

100

137

56

41

17

12

5.0

6

2.4

6.0

2.5

2.4

1

Average

57.2

17.4

6.1

2.6

2.6

1

SD

3.4

0.8

1.0

0.5

0.2

C

Translation / post translation efficiency (Lucieferase / mRNA)

MOI

Cell lines

EVSA-T

CAMA-1

MCF-7

T47 D

SKBR-3

ZR 75.1

1

2

1

2

1

3

1.2

4

1.6

4

1.6

2.4

1

10

6

1

6

1

6

1.0

8

1.4

6

1.0

6.0

1

100

26

2

38

2

33

2.0

15

0.9

13

0.8

16.2

1

Average

1.2

1.4

1.4

1.3

1.1

1

SD

0.4

0.8

0.6

0.4

0.4

D

Luciferase activity

MOI

Cell lines

EVSA-T

CAMA-1

MCF-7

T47 D

SKBR-3

ZR 75.1

1

28

50

10

18

5

8

2

4

2

4.4

1

1

10

620

74

167

20

65

8

32

4

22

2.7

8

1

100

30473

105

13171

46

3790

13

648

2

616

2.1

289

1

Average

76.4

28

9.7

3.2

3.0

1

SD

27.8

15

2.9

0.8

1.2