Figure 10

Luciferase activity and viral DNA in human breast cancer cell lines. A: Luciferase activity measured in cell lysate 48 hours after infection at different MOI. B: Number of Adenoviral DNA copies per cell determined by duplex real time PCR. The number of cells was determined by microscopic counting and the number of genome by real time PCR of the GAPDH gene. All the cell lines had similar ploidy. C: Quantification of the luciferase mRNA in the cell lines after infection at different MOI by Northern blotting. The density of the bands was determined with a phosphorimager. The β-actin blotting was also performed afterwards to control the quality of the RNA extraction and the quantity of RNA loaded. The density of the β-actin bands was similar in all the cell lines (data not shown). Values are means of a triplicate ± SD.